Hello all I am going to enter the Healthcare innovation contest this
year. My idea is based on the fact that the flu virus and other
respiratory viruses activate interferon's that result in cell death by
cytotoxic T-cells reducing the cell count in the nasal cavity, and the
fact that bacterial infections could have he opposite effect because of
the emergence of macrophages to clear the infection. It has also been
noted that bacterial infection due to the clearing of mucus secreting
epithelial cells is a rare but could cause problems in this test. I am
going to preform an experiment to test this hypothesis. I will be using
nasopharyngeal, and sputum specimens to preform cell counts on
individuals with confirmed influenza, on healthy patients, and on
patients with a bacterial infection like strep A. I will preform the
cell count with a Wright stain and a high powered light microscope. I
will then compare the positive flu sputum to the healthy and bacterial
infected sputum, and will do the same with the nasopharyngeal
specimens. I will try to get a minimum of thirty samples so that my
error rate can be within three significant figures. If my hypothesis is
correct this can be used as an at home or field screening tool for
respiratory viral and bacterial infections by using the cell phone
cytometer developed by by Aydogan Ozcan’s group at University of
California, Los Angeles (Anal. Chem., Article ASAP DOI: 10.1021/ac201587a) and described by this article http://www.labtimes.org/labtimes/trick/tricks/2011_05.lasso
My
research can also lead to a cheaper method of screening for respiratory
viral and bacterial infections by removing the need for costly test
kits and instrumentation and instead using already established
instrumentation like the iris from iris diagnostic. Some might say that
the viscosity of sputum is too great and will clog the instrument but
this can easily be overcome with the use of sputolysum.
here is a link to the contest I am entering http://ceshealth.com/
References:
http://www.medicinenet.com/interferon/article.htm
http://pathmicro.med.sc.edu/mhunt/flu.htm
http://micro.magnet.fsu.edu/cells/viruses/influenzavirus.html
http://www.labtimes.org/labtimes/trick/tricks/2011_05.lasso
http://www.biomedcentral.com/1471-2334/10/340?fmt_view=mobile
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